Professor Layton (voices of Christopher Robin Miller / Yô Ôizumi), true English gentleman and the world's greatest amateur super sleuth embarks on his most daring adventure yet when he receives a letter from his old student, the famous opera diva Janice Quatlane (voices of Emma Tate / Nana Mizuki). She is to perform at the legendary Crown Petone Opera House and invites him to attend as her special guest. Meanwhile, a spate of disappearances hits London. Two young school girls are the latest victims and the Professor suspects it's related to the mysterious occurrences at the theatre. The Professor and his loyal assistant Luke (voices of Maria Darling / Maki Horikita) travel to the Opera House to solve their toughest puzzle yet, the mystery of Eternal Life!
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